Spectrophotometry and Spectrofluorimetry : A Practical Approach / edited by Michael G. Gore.
Material type:
TextSeries: The Practical Approach SeriesPublication details: Oxford : Oxford University Press, c.2000.Description: xvii, 368 p. : ill. ; 25 cmISBN: - 0199638128 (pbk. : alk. paper)
- 572.36 GOR
| Item type | Current library | Call number | Copy number | Status | Date due | Barcode | |
|---|---|---|---|---|---|---|---|
| Standard Loan | Moylish Library Main Collection | 572.36 GOR (Browse shelf(Opens below)) | 1 | Available | 39002100322594 |
Enhanced descriptions from Syndetics:
Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.
Includes bibliographical references and index.
Introduction to light absorption : visible and ultraviolet spectra / Robert K. Poole and Uldis Kalnenieks -- Fluorescence principles and measurement / Arthur G. Szabo -- Time-resolved fluorescence and phosphorescence spectroscopy / Thomas D. Bradrick and Jorge E. Churchich -- Introduction to circular dichroism / Alison Rodger and Matthew A. Ismail -- Quantitative determination of equilibrium binding isotherms for multiple ligand-macromolecule interactions using spectroscopic methods / Wlodzimierz Bujalowski and Maria J. Jezewska -- Steady-state kinetics / Athel Cornish-Bowden --Spectrophotometric assays / T.J. Mantle and D.A. Harris -- Stopped-flow spectroscopy / M.T. Wilson and J. Torres -- Stopped-flow fluorescence spectroscopy / Michael G. Gore and Stephen P. Bottomley -- Stopped-flow circular dichroism / Alison Rodger and Michael J. Carey -- Spectrophotometry and fluorimetry of cellular compartments and intracellular processes / C. Lindsay Bashford -- Use of optical spectroscopic methods to study the thermodynamic stability of proteins / Maurice R. Eftink and Haripada Maity -- The use of spectroscopic techniques in the study of DNA stability / John SantaLucia, Jr.
Table of contents provided by Syndetics
- List of protocols
- Abbreviations
- 1 Introduction to light absorption visible and ultraviolet spectra
- 2 Fluorescence principles and measurement
- 3 Time resolved fluorescence and phosphorescence spectroscopy
- 4 An introduction to circular dichroism
- 5 Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
- 6 Steady state kinetics
- 7 Spectrophotometric assays
- 8 Stopped flow spectroscopy
- 9 Stopped flow fluorescence spectroscopy
- 10 Stopped flow circular dichroism
- 11 Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
- 12 Use of optical spectroscopic methods to study the thermodynamic stability of proteins
- 13 The use of spectroscopic techniques in the study of DNA stability
- List of suppliers
- Index