Molecular forensics / edited by Ralph Rapley, David Whitehouse.

By:

Rapley, Ralph

Contributor(s):

Material type: Text

TextPublication details: Chichester, England ; Hoboken, NJ : John Wiley & Sons, c2007.Description: xiii, 244 p., [8] p. of plates : ill. (some col.) ; 25 cmISBN:

9780470024966 (pbk. : alk. paper)
0470024968 (pbk. : alk. paper)

Subject(s):

DDC classification:

363.25 RAP

Online resources:

Table of contents only

Contents:

Current and future trends in forensic molecular biology / Simon J. Walsh -- Basic tools and techniques in molecular biology / Ralph Rapley and David Whitehouse -- Automated DNA extraction techniques for forensic analysis / Marion Nagy -- Real-time quantitative PCR in forensic science / Antonio Alonso and Oscar García -- Minisatellite and microsatellite DNA typing analysis / Keiji Tamaki -- Application of SNPs in forensic casework / Claus Børsting, Juan J. Sanchez and Niels Morling -- The X chromosome in forensic science : past, present and future / Reinhard Szibor -- Mitochondrial analysis in forensic science / Hirokazu Matsuda and Nobuhiro Yukawa -- Y-chromosomal markers in forensic genetics / Manfred Kayser -- Laser microdissection in forensic science / Luigi Saravo ... [et al.] -- Laboratory information systems for forensic analysis of DNA evidence / Benoît Leclair and Tom Scholl -- Statistical presentation of forensic data / Mark A. Best -- Protein profiling for forensic and biometric applications / Mikhail Soloviev ... [et al.] -- Application of MRS in forensic pathology / Eva Scheurer, Michael Ith, and Chris Boesch.

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Standard Loan	Moylish Library Main Collection	363.25 RAP (Browse shelf(Opens below))	1	Available		39002100366542

Enhanced descriptions from Syndetics:

Molecular Forensics offers a comprehensive coverage of the increasingly important role that molecular analysis plays within forensic science. Starting with a broad introduction of modern forensic molecular technologies, the text covers key issues from the initial scenes of crime sampling to the use of evidential material in the prosecution of legal cases. The book also explores the questions raised by the growing debate on the applications of national DNA databases and the resulting challenges of developing, maintaining and curating such vast data structures. The broader range of applications to non-human cases is also discussed, as are the statistical pitfalls of using so-called unique data such as DNA profiles, and the ethical considerations of national DNA databases.

An invaluable reference for students taking courses within the Forensic and Biomedical sciences, and also useful for practitioners in the field looking for a broad overview of the subject.

Provides a comprehensive overview of modern forensic molecular technologies. Explores the growing debate on the applications of national DNA databases. Discusses the initial phases of investigation to the conclusion of cases involving molecular forensic analysis.

Includes bibliographical references and index.

Table of contents provided by Syndetics

Preface (p. ix)
List of Contributors (p. xi)
1 Current and Future Trends in Forensic Molecular Biology (p. 1)
1.1 Introduction (p. 1)
1.2 Developments within the field of forensic molecular biology (p. 2)
1.3 Developments influencing law enforcement - operational impacts (p. 7)
1.4 Developments influencing the justice system - socio-legal impacts (p. 10)
1.5 Summary (p. 11)
1.6 References (p. 12)
2 Basic Tools and Techniques in Molecular Biology (p. 21)
2.1 Introduction (p. 21)
2.2 Isolation and separation of nucleic acids (p. 21)
2.3 Automated analysis of nucleic acid fragments (p. 23)
2.4 Molecular biology and bioinformatics (p. 24)
2.5 The polymerase chain reaction (PCR) (p. 25)
2.6 Applications of the PCR (p. 28)
2.7 Nucleotide sequencing of DNA (p. 30)
2.8 Conclusion (p. 34)
2.9 References (p. 34)
3 Automated DNA Extraction Techniques for Forensic Analysis (p. 37)
3.1 Introduction (p. 37)
3.2 Principal steps of DNA extraction (p. 38)
3.3 DNA extraction techniques (p. 39)
3.4 Modified techniques for DNA extraction from challenging forensic samples (p. 43)
3.5 Automation of DNA extraction (p. 45)
3.6 References (p. 56)
4 Real-time Quantitative PCR in Forensic Science (p. 59)
4.1 Introduction (p. 59)
4.2 Current real-time PCR chemistries (p. 60)
4.3 Human nuclear DNA quantification (p. 61)
4.4 Human mitochondrial DNA quantification (p. 65)
4.5 Detection and quantification of non-human species (p. 66)
4.6 Concluding remarks and perspectives (p. 67)
4.7 References (p. 67)
5 Minisatellite and Microsatellite DNA Typing Analysis (p. 71)
5.1 Introduction (p. 71)
5.2 Minisatellites (p. 71)
5.3 Microsatellites (p. 80)
5.4 Acknowledgements (p. 86)
5.5 References (p. 86)
6 Application of SNPs in Forensic Casework (p. 91)
6.1 Introduction (p. 91)
6.2 Single nucleotide polymorphisms (p. 92)
6.3 Single nucleotide polymorphism typing technology (p. 94)
6.4 Single nucleotide polymorphisms for human identification (p. 95)
6.5 Single nucleotide polymorphisms in mitochondrial DNA (p. 98)
6.6 Forensic DNA phenotyping (p. 98)
6.7 Ethical considerations of SNP genotyping (p. 100)
6.8 References (p. 100)
7 The X Chromosome in Forensic Science: Past, Present and Future (p. 103)
7.1 Introduction (p. 103)
7.2 History of forensic utilization of the X chromosome (p. 104)
7.3 Chromosome X short tandem repeats (p. 107)
7.4 Power of ChrX markers in trace analysis (p. 111)
7.5 Power of ChrX markers in kinship testing (p. 111)
7.6 Chromosome X marker mapping and haplotype analysis (p. 114)
7.7 Chromosome X-chromosome Y homologue markers (p. 119)
7.8 Chromosome X STR allele and haplotype distribution in different populations (p. 119)
7.9 Ethical considerations in ChrX marker testing (p. 120)
7.10 Concluding remarks (p. 121)
7.11 References (p. 121)
8 Mitochondrial Analysis in Forensic Science (p. 127)
8.1 Introduction (p. 127)
8.2 Mitochondrial DNA (mtDNA) biology (p. 128)
8.3 Identification of individuals (mtDNA typing) (p. 132)
8.4 Topics of forensic interest (p. 134)
8.5 References (p. 138)
9 Y-Chromosomal Markers in Forensic Genetics (p. 141)
9.1 Introduction (p. 141)
9.2 Identification of the male sex (p. 142)
9.3 Identification of male lineages (p. 143)
9.4 Identification of a male's paternity (p. 148)
9.5 Identification of a male's geographical origin (p. 151)
9.6 The future of Y-chromosomal markers in forensics (p. 155)
9.7 Acknowledgements (p. 156)
9.8 References (p. 157)
10 Laser Microdissection in Forensic Science (p. 163)
10.1 Introduction (p. 163)
10.2 Histological, biochemical analysis (p. 166)
10.3 References (p. 169)
11 Laboratory Information Systems for Forensic Analysis of DNA Evidence (p. 171)
11.1 Introduction (p. 171)
11.2 The specifications of forensic genotyping assays (p. 173)
11.3 Automated pipetting (p. 174)
11.4 Analysis of STR data (p. 176)
11.5 Bioinformatics (p. 178)
11.6 Conclusion (p. 180)
11.7 References (p. 181)
12 Statistical Presentation of Forensic Data (p. 185)
12.1 Introduction (p. 185)
12.2 Techniques (p. 185)
12.3 Laboratory issues (p. 188)
12.4 Statistical analysis (p. 189)
12.5 Other issues (p. 193)
12.6 Special situations (p. 194)
12.7 References (p. 195)
13 Protein Profiling for Forensic and Biometric Applications (p. 197)
13.1 Introduction (p. 197)
13.2 Protein assays in molecular forensics: current status (p. 197)
13.3 Novel technologies and the remaining challenges (p. 204)
13.4 Protein markers for use in forensic and biometric applications (p. 208)
13.5 References (p. 217)
14 Application of MRS in Forensic Pathology (p. 221)
14.1 Forensic, criminalistic and ethical significance of time of death (p. 221)
14.2 Classical methods for the determination of PMI (p. 222)
14.3 Magnetic resonance spectroscopy (p. 224)
14.4 How to predict PMI based on MRS measurements (p. 229)
14.5 Outlook (p. 232)
14.6 References (p. 235)
Index (p. 241)

Author notes provided by Syndetics

Dr Ralph Rapley, Department of Biosciences, University of Hertfordshire, Hatfield, UK

Dr David Whitehouse, Consultant Biotechnologist and Research Fellow, London School of Hygiene and Tropical Medicine, UK