Diagnostic molecular pathology : a practical approach : volume I : phenotyping and genotyping of intact cells / edited by C. S. Herrington and J. O'D McGee

Enhanced descriptions from Syndetics:

Molecular techniques are now being widely applied in clinical pathology laboratories, yet many people working in these facilities have only limited experience of such procedures. Diagnostic Molecular Pathology volumes I and II bring together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples.Topics covered in Volume I include those methods which relate to morphological analysis, such as immunocytochemistry, in situ hybridization, chromosome analysis, and flow cytometry. Volume II demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing, and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies.For all the protocols there is expert guidance and detailed instruction on how to ensure accuracy and reliability, which will appeal to people working at all levels in pathology.

Table of contents provided by Syndetics

• 1 Extraction of Nucleic Acids from Clinical Samples and Cultured Cells
• 2 Probe Preparation and Labelling
• 3 Molecular Hybridization of Nucleic Acids
• 4 The Polymerase Chain Reaction and the Molecular Genetic Analysis of Tissue Biopsies
• 5 DNA Sequencing
• 6 Determination of Genital Human Papillomavirus Infection of Cells and Tissues by Generic Polymerase Chain Reaction Amplification
• 7 The Polymerase Chain Reaction for Human Papillomavirus Screening in Diagnostic Cytopathology of the Cervix
• 8 Molecular Detection and Identification of Pathogenic Organisms
• 9 Human Identification by DNA Analysis: The Amplification Fragment Length Polymorphism (AMPFLP) Analysis Technique
• 10 Analysis of Archival Material: Application to Tumor Pathology

Author notes provided by Syndetics