Immunochemistry labfax / edited by M.A. Kerr and R. Thorpe.

Includes bibliographical references and index.

Plasma proteins -- Plasma and serum -- Anticoagulants -- Serum -- Plasma/serum proteins -- Factors affecting the concentration of plasma proteins -- Effect of age -- Acute phase response -- Plasma proteins in other body fluids -- Antibodies recognizing plasma/serum proteins -- Absorption of unwanted specificities -- Anti-whole sera antibodies -- Purified antibodies -- Storage of commercial antibodies -- Properties of immunoglobulins -- Diversity of immunoglobulins -- Human immunoglobulin allotypes -- Immunoglobulins from other species -- Immunoglobulin receptors (Fc receptors) -- Antibodies recognizing immunoglobulins -- Specificity of antibodies recognizing immunoglobulins of other species -- Producting antibodies -- Polyclonal antisera -- Monoclonal antibodies -- rDNA-produced antibodies -- Purification and fragmentation of immunoglobulins -- General properties of antibodies -- Sources of immunoglobulins -- Methods of purification of IgG -- Purification of IgM -- Purification of serum IgA -- Other techniques for immunoglobulin or antibody purification.

(cont) Properties of bacterial IgG-binding proteins: protein A and protein G -- Properties of IgA-binding proteins -- Purified immunoglobulins available commercially -- Preparation of immunoglobulin fragments -- Preparation and use of radiolabeled antibodies and antigens -- Labeling with radioisotopes -- Labeling with other isotopes -- Radioimmunoassay -- Immunoradiometric assay -- Enzyme-conjugated antibodies: preparation and use -- Enhanced enzyme immunodetection systems -- Enzymes commonly used in immunoconjugates -- Methods for the conjugation of enzymes to antibodies -- Properties of enzyme substrates commonly used in immunoassay -- Derivatization of antibodies and antigens: fluorescent labeling, biotinylation, immunogold derivatives -- Fluorescently labeled antibodies -- Biotinylation of proteins -- Colloidal gold derivatives -- Common immunological techniques: elisa, blotting, immunohistochemistry and immunocytochemistry -- ELISA -- Immunoblotting -- Immunohistochemistry and immunocytochemistry -- Complement -- Nomenclature -- General properties of complement -- Proteins of the complement system.

(cont) Control of the complement system -- Fragmentation of C3 -- Complement receptors -- Detection and estimation of complement components -- Antigenic assays for complement components -- Functional assays and enzyme assays -- Detection of activation products -- Biological assays -- Complement measurement in disease -- Safety -- Chemical safety -- Radiochemical safety -- Microbiological safety -- Viral safety.