Mass spectrometry in sports drug testing : characterization of prohibited substances and doping control analytical assays / Mario Thevis.

By:

Thevis, Mario, 1973-

Material type: Text

TextSeries: Wiley-interscience series in mass spectrometryPublication details: Oxford : Wiley, 2010.Description: xiii, 360 p. : ill. ; 24 cmISBN:

0470413271 (hbk.)
9780470413272 (hbk.)

Subject(s):

DDC classification:

362.29 THE

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Standard Loan	Moylish Library Main Collection	362.29 THE (Browse shelf(Opens below))	1	Available		39002100402263

Enhanced descriptions from Syndetics:

Enables you to detect, identify, and characterize hundreds of drugs that may be used by athletes

Mass spectrometry has become essential to sports drug testing. This book examines both the principles of sports drug testing and the use of mass spectrometry techniques and mass spectral data to detect, identify, and characterize hundreds of known and unknown drugs that athletes may use to enhance their performance. The author provides a detailed overview of the mass spectrometry of numerous classes of therapeutics and agents, various analyzers to detect low- and high-molecular weight drugs, as well as techniques to discriminate between endogenously produced and synthetically derived compounds.

Mass Spectrometry in Sports Drug Testing begins with a full chapter dedicated to the history of sports drug testing. Next, the book provides the principles and techniques needed to maximize the specificity and sensitivity of mass spectrometric assays, including:

Detailed, step-by-step assays with sample preparation Discussion of both chromatographic separation and mass spectrometric analysis Characterization of analytes in order to unequivocally identify banned substances Mass spectrometric behavior of low- and high-molecular weight analytes

Throughout the book, descriptive examples illustrate the principles, advantages, and limitations of different assays.

Mass Spectrometry in Sports Drug Testing not only sets forth the role mass spectrometry plays in detecting drug use among athletes, it also adds new insights into the health and ethical issues of doping in sports.

Includes bibliographical references and index.

Table of contents provided by Syndetics

Preface (p. xi)
Acknowledgments (p. xiii)
1 History of Sports Drug Testing (p. 1)
1.1 Historical Attempts of Artificial Performance Enhancement (p. 1)
1.2 Background and Rationale of Doping Controls (p. 2)
1.2.1 Cheating (p. 3)
1.2.2 Health Issues (p. 3)
1.2.3 Ethical Issues (p. 3)
1.3 Early Detection Methods: Possibilities and Limitations of Assays Without Mass Spectrometry (p. 4)
1.3.1 First Applications Using Chemical and Biological Approaches in Horse Doping Control (p. 4)
1.3.2 First Applications Using Chemical Approaches in Human Doping Control (p. 8)
1.4 Introduction of Mass Spectrometry to Doping Control Analysis (p. 15)
1.4.1 First Approaches and Adverse Analytical Findings (p. 16)
1.4.2 Progression of Analytical Methods (p. 21)
References (p. 24)
2 Mass Spectrometry and the List of Prohibited Substances and Methods of Doping (p. 44)
2.1 Criteria for the Mass Spectrometric Identification of Prohibited Compounds (p. 44)
2.1.1 Low Molecular Weight Analytes (p. 45)
2.1.2 High Molecular Weight Analytes (p. 46)
2.2 Modern Mass Spectrometers in Doping Controls: Advantages and Disadvantages of Available Techniques (p. 48)
2.2.1 Ionization Techniques in Routine Doping Controls (p. 48)
2.2.2 Mass Analyzers in Routine Doping Controls (p. 53)
References (p. 60)
3 Structure Characterization of Low Molecular Weight Target Analytes-Electron Ionization (p. 70)
3.1 Stimulants (p. 70)
3.2 Narcotics (p. 81)
3.3 Anabolic Androgenic Steroids (p. 87)
3.3.1 Unsaturated 3-Keto-Steroids (p. 88)
3.3.2 ¿, ß-Saturated Ketosteroids (p. 92)
3.3.3 3-Keto-1, 4-Diene, 3-Keto-4, 6-Diene, and 3-Keto-4,9(11)-Diene Steroids (p. 95)
3.3.4 Steroid Derivatization (p. 95)
3.4 Selective Androgen Receptor Modulators (SARMs) (p. 99)
3.4.1 2-Quinolinone-Based SARMs (p. 100)
3.4.2 Hydroxybicyclic Hydantoin-Derived SARMs (p. 100)
3.5 Diuretics (p. 104)
3.5.1 Thiazide-Derived Drugs (p. 104)
3.5.2 Benzoic Acid-Derived Loop Diuretics (p. 106)
3.5.3 Potassium-Sparing Diuretics (p. 106)
3.6 ß 2 -Agonists (p. 110)
3.7 ß-Receptor Blocking Agents (p. 114)
3.8 Calcium-Channel Modulators (RYCALS) (p. 118)
3.9 Carbohydrate-Based Agents (p. 122)
3.9.1 Mannitol (p. 122)
3.9.2 Glycerol (p. 125)
3.9.3 Hydroxyethyl Starch and Dextran (p. 127)
References (p. 130)
4 Structure Characterization of Low Molecular Weight Target Analytes: Electrospray Ionization (p. 148)
4.1 Stimulants (p. 149)
4.2 Narcotics (p. 155)
4.3 Anabolic Androgenic Steroids (p. 159)
4.3.1 ¿, ß-Saturated 3-Keto-Steroids (p. 159)
4.3.2 3-Keto-4-ene and 3-Keto-1-ene Steroids (p. 164)
4.3.3 3-Keto-1,4-diene Steroids (p. 165)
4.3.4 3-Keto-4,6-diene Steroids (p. 167)
4.3.5 3-Keto-4,9-diene Steroids (p. 167)
4.3.6 3-Keto-4,9,11-triene Steroids (p. 167)
4.3.7 Stanozolol (p. 168)
4.4 Selective Androgen Receptor Modulators (SARMs) (p. 171)
4.4.1 Arylpropionamide-Derived SARMs (p. 173)
4.4.2 Hydroxybicyclic Hydantoin-Derived SARMs (p. 174)
4.4.3 2-Quinolinone-Derived SARMs (p. 178)
4.4.4 Tetrahydroquinoline-Derived SARMs (p. 178)
4.5 Diuretics (p. 181)
4.5.1 Thiazide-Derived Drugs (p. 181)
4.5.2 Benzoic Acid-Derived Loop Diuretics (p. 182)
4.5.3 Potassium-Sparing Diuretics (p. 186)
4.6 ß 2 -Agonists (p. 187)
4.7 Calcium-Channel Modulators (RYCALS) (p. 191)
4.8 Peroxisome-Proliferator Activated Receptor-¿ (PPAR¿) And Adenosine Monophosphate Activated Protein Kinase (AMPK) Agonists (p. 193)
4.9 Hypoxia-Inducible Factor (HIF)-Stabilizers And Sirtuin Activators (p. 198)
4.9.1 HIF-Stabilizers (p. 198)
4.9.2 Sirtuin Activators (p. 202)
4.10 ß-Receptor Blocking Agents (p. 207)
4.11 Glucuronic Acid and Sulfate Conjugates of Target Analytes (p. 212)
References (p. 214)
5 Structure Characterization of High Molecular Weight Target Analytes: Electrospray Ionization (p. 225)
5.1 Human Chorionic Gonadotrophin (hCG) (p. 225)
5.2 Erythropoietins (EPO) (p. 227)
5.3 Synacthen (p. 230)
5.4 Insulins (p. 233)
5.5 Hemoglobin-Based Oxygen Carriers (HBOCs) (p. 237)
5.6 Human Growth Hormone (hGH) (p. 242)
5.7 Sermorelin (Geref) (p. 249)
5.8 Insulin-Like Growth Factor-1 (IGF-1) (p. 251)
5.9 Gonadorelin (LHRH) (p. 255)
References (p. 256)
6 Modern Mass Spectrometry-Based Analytical Assays (p. 275)
6.1 GC-MS and Isotope Ratio Mass Spectrometry (p. 276)
6.1.1 Stimulants/Narcotics (p. 276)
6.1.2 Anabolic Androgenic Steroids (p. 285)
6.1.3 Diuretics (p. 303)
6.1.4 ß 2 -Agonists and ß-Receptor Blocking Agents (p. 306)
6.1.5 Carbohydrate-Based Agents (p. 307)
6.2 LC-MS/MS (p. 310)
6.2.1 Stimulants/narcotics/ß 2 -Agonists/ß-Receptor Blocking Agents (p. 311)
6.2.2 Anabolic Androgenic Steroids/Corticosteroids/Hormone Antagonists and Modulators (p. 313)
6.2.3 Masking Agents (Including Diuretics) (p. 315)
6.2.4 Peptide Hormones (p. 316)
6.2.5 Hemoglobin-Based Oxygen Carriers (p. 318)
6.2.6 Human Chorionic Gonadotrophin (hCG) (p. 320)
References (p. 322)
7 Limitations and Perspectives of Mass Spectrometry-Based Procedures in Doping Control Analysis (p. 340)
7.1 Recombinant Biomolecules (p. 340)
7.1.1 Erythropoietin (p. 340)
7.1.2 Human Growth Hormone (p. 341)
7.1.3 Human Insulin and IGF-1 (p. 342)
7.2 Unknown Compounds (p. 342)
7.3 Profiling of Urine and/or Blood (p. 343)
7.4 Alternative Specimens (p. 344)
References (p. 345)
Index (p. 351)

Author notes provided by Syndetics

Mario Thevis, PhD, is Professor for Preventive Doping Research at the German Sport University Cologne. He has published more than 115 articles in peer-reviewed journals, mainly on mass spectrometry and sports drug testing. Dr. Tevis is often invited to national and international conferences as a speaker and keynote lecturer on mass spectrometry and doping controls.